Chimpanzee, Baboon, Orangutan
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human BUD13 according to NP_116114.1
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH: 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
Use at 2-10 µg/mg of lysate.
Anti-BUD13 antibody 图像
Western blot - Anti-BUD13 antibody (ab117823)
All lanes : Anti-BUD13 antibody (ab117823) at 0.1 µg/ml Lane 1 : Whole HeLa cell lysate at 50 µg Lane 2 : Whole HeLa cell lysate at 15 µg Developed using the ECL technique Predicted band size : 71 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-BUD13 antibody (ab117823)
Detection of Human BUD13 by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab117823 at 6 µg/mg of lysate. Subsequent WB detection was done using ab117823 at 1 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-BUD13 antibody (ab117823)参考文献
has not yet been referenced specifically in any publications.
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