概述

  • 产品名称Anti-Bub3抗体
    参阅全部 Bub3 一抗
  • 描述
    兔多克隆抗体to Bub3
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 275 and 325 of Human Bub3 (Swiss-Prot O43684).

  • 阳性对照
    • Human metastatic lymph node. Human prostate adenocarcinoma.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
    • 序列相似性Belongs to the WD repeat BUB3 family.
      Contains 5 WD repeats.
    • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Aa2-050 antibody
      • AU019800 antibody
      • AU021329 antibody
      • AU043350 antibody
      • AW146323 antibody
      • BUB 3 antibody
      • BUB 3L antibody
      • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody
      • bub3 antibody
      • BUB3 budding uninhibited by benzimidazoles 3 homolog antibody
      • BUB3 mitotic checkpoint protein antibody
      • BUB3_HUMAN antibody
      • BUB3L antibody
      • Budding uninhibited by benomyl antibody
      • Budding uninhibited by benzimidazoles 3 antibody
      • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody
      • Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody
      • Budding uninhibited by benzimidazoles 3 homolog antibody
      • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody
      • C78067 antibody
      • hBUB3 antibody
      • Mitotic checkpoint component antibody
      • Mitotic checkpoint protein bub3 antibody
      • OTTHUMP00000020679 antibody
      • OTTHUMP00000020680 antibody
      • WD repeat type I transmembrane protein A72.5 antibody
      see all

    Anti-Bub3 antibody 图像

    • ab84448, at 1/250 dilution, staining Bub3 in formalin-fixed, paraffin-embedded human metastatic lymph node tissue by Immunohistochemistry using DAB detection.
    • ab84448, at 1/250 dilution, staining Bub3 in formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue by Immunohistochemistry using DAB detection.

    Anti-Bub3 antibody (ab84448)参考文献

    ab84448 has not yet been referenced specifically in any publications.

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