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Our Abpromise guarantee covers the use of ab9000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/2000. Predicted molecular weight: 122 kDa.|
Western blot using ab9000 on Bub1 antigen (lanes 1- 3), and HL-60 whole cell extract.
The Bub1 antigen is only a fragment of full length Bub1 and thus runs at a lower size than the band seen in HL-60 extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"