Anti-Bu1b抗体[5-11G2] (Phycoerythrin) (ab25434)

概述

  • 产品名称Anti-Bu1b抗体[5-11G2] (Phycoerythrin)
    参阅全部 Bu1b 一抗
  • 描述
    小鼠单克隆抗体[5-11G2] to Bu1b (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性This antibody is specific to Chicken Bu1b alloantigen, Mr 70 kDa. The antibody does not react with cells from CHA and H.B15Ab strains by immunofluorescence.
  • 经测试应用适用于: Flow Cyt, IP, IHC-Frmore details
  • 种属反应性
    与反应: Chicken
  • 免疫原

    Tissue/ cell preparation: Bursal cells from 2 to 5 week old CHA (Bu1b) strain chickens.

  • 阳性对照
    • Chicken peripheral blood mononuclear cells for Immunofluorescent staining and flow cytometric analysis.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS; Stabilizing agent
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号5-11G2
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IP
IHC-Fr
  • 应用说明Flow Cyt: Use up to 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.
    • 细胞定位Cell membrane
    • 数据库链接
    • 别名
      • B6.1 antibody
      • B6.2 antibody
      • Bu 1b antibody
      • LOC396098 antibody

    Anti-Bu1b antibody [5-11G2] (Phycoerythrin) (ab25434)参考文献

    ab25434 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab25434.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"