Anti-Bu1a + Bu1b抗体[AV20] (Phycoerythrin) (ab25437)

概述

  • 产品名称Anti-Bu1a + Bu1b抗体[AV20] (Phycoerythrin)
    参阅全部 Bu1a + Bu1b 一抗
  • 描述
    小鼠单克隆抗体[AV20] to Bu1a + Bu1b (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性ab25437 recognises Bu1a and Bu1b.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Chicken
    不与反应: Turkey
  • 免疫原

    Tissue/ cell preparation: Bursal cells from one day old H.B15 (Bu 1a/b) strain chickens.

性能

应用

Our Abpromise guarantee covers the use of ab25437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IHC-Fr
IP
  • 应用说明Flow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Note: samples need to be acetone fixed.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance. Bu1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
    • 细胞定位Type I membrane protein.
    • 数据库链接
    • 别名
      • B6.1 antibody
      • B6.2 antibody
      • Bu 1a antibody
      • Bu 1b antibody
      • LOC396098 antibody
      see all

    Anti-Bu1a + Bu1b antibody [AV20] (Phycoerythrin) (ab25437)参考文献

    This product has been referenced in:
    • Rothwell CJ  et al. Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol 55:225-34 (1996). Read more (PubMed: 9014319) »

    See 1 Publication for this product

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