概述

  • 产品名称Anti-Bu1a + Bu1b抗体[AV20]
    参阅全部 Bu1a + Bu1b 一抗
  • 描述
    小鼠单克隆抗体[AV20] to Bu1a + Bu1b
  • 经测试应用适用于: IP, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Chicken
    不与反应: Turkey
  • 免疫原

    Tissue/ cell preparation: Bursal cells from one day old H.B15 (Bu1a/b) strain chickens.

性能

应用

Our Abpromise guarantee covers the use of ab25400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with acetone
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性The Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance. Bu1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
  • 细胞定位Type I membrane protein.
  • 数据库链接
  • 别名
    • B6.1 antibody
    • B6.2 antibody
    • Bu 1a antibody
    • Bu 1b antibody
    • LOC396098 antibody
    see all

Anti-Bu1a + Bu1b antibody [AV20] (ab25400)参考文献

This product has been referenced in:
  • Bradaric MJ  et al. Immune Cells in the Normal Ovary and Spontaneous Ovarian Tumors in the Laying Hen (Gallus domesticus) Model of Human Ovarian Cancer. PLoS One 8:e74147 (2013). IHC-P, Flow Cyt ; Chicken . Read more (PubMed: 24040191) »
  • Rothwell CJ  et al. Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol 55:225-34 (1996). Read more (PubMed: 9014319) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"