Anti-BTN2A2/BTF2抗体[6C7D2] (ab233763)
Key features and details
- Mouse monoclonal [6C7D2] to BTN2A2/BTF2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-BTN2A2/BTF2抗体[6C7D2]
参阅全部 BTN2A2/BTF2 一抗 -
描述
小鼠单克隆抗体[6C7D2] to BTN2A2/BTF2 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human BTN2A2/BTF2 aa 57-237. (Expressed in E.coli).
Database link: Q8WVV5 -
阳性对照
- WB: Recombinant human BTN2A2/BTF2 protein; BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate. Flow: HeLa cells. IHC-P: Cervical cancer and bladder cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
6C7D2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab233763于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
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说明 |
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WB
1/500 - 1/2000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
靶标
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组织特异性
Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10385 Human
- Omim: 613591 Human
- SwissProt: Q8WVV5 Human
- Unigene: 373938 Human
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别名
- BT2.2 antibody
- BT2A2_HUMAN antibody
- BTF2 antibody
see all
图片
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Paraffin-embedded human bladder cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human cervical cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling BTN2A2/BTF2 using ab233763 at 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate
Developed using the ECL technique. -
Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution + Recombinant human GCSF receptor (AA: extra 25-187) protein
Expected MW is 46.4 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab233763 尚未被引用在任何文献中。