RabMAb

Anti-BTK (phospho Y551)抗体[EP267Y] (ab40770)

概述

  • 产品名称Anti-BTK (phospho Y551)抗体[EP267Y]
    参阅全部 BTK 一抗
  • 描述
    兔单克隆抗体[EP267Y] to BTK (phospho Y551)
  • 特异性The antibody will detect BTK phosphorylation on Tyrosine 551.
  • 经测试应用适用于: WB, ICCmore details
    不适用于: Flow Cyt,IHC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BTK.

  • 阳性对照
    • K562 cell lysate, Jurkat cell lysate (Pervanadate treated)
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab40770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt,IHC or IP.
  • 靶标

    • 功能Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
    • 疾病相关Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
      Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 Btk-type zinc finger.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 翻译后修饰Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
    • 细胞定位Cytoplasm. Membrane. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Agammaglobulinaemia tyrosine kinase antibody
      • AGMX 1 antibody
      • AGMX1 antibody
      • AT antibody
      • ATK antibody
      • B cell progenitor kinase antibody
      • B-cell progenitor kinase antibody
      • BPK antibody
      • Bruton agammaglobulinemia tyrosine kinase antibody
      • Bruton tyrosine kinase antibody
      • Bruton’s Tyrosine Kinase antibody
      • Btk antibody
      • BTK_HUMAN antibody
      • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
      • IMD 1 antibody
      • IMD1 antibody
      • MGC126261 antibody
      • MGC126262 antibody
      • OTTHUMP00000063593 antibody
      • PSCTK 1 antibody
      • PSCTK1 antibody
      • truncated Bruton agammaglobulinemia tyrosine kinase antibody
      • Tyrosine protein kinase BTK antibody
      • Tyrosine-protein kinase BTK antibody
      • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
      • XLA antibody
      see all

    Anti-BTK (phospho Y551) antibody [EP267Y] 图像

    • All lanes : Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770) at 1/5000 dilution

      Lane 1 : Ramos (human Burkitt's lymphoma) treated with Pervanadate whole cell lysates The treatment protocols were not recorded.
      Lane 2 : Untreated Ramos (human Burkitt's lymphoma) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 74 kDa
      Observed band size : 74 kDa


      Exposure time : 30 seconds

      Blocking/Diluting buffer 5% NFDM/TBST

    Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)参考文献

    This product has been referenced in:
    • Rushworth SA  et al. Identification of Bruton's tyrosine kinase as a therapeutic target in acute myeloid leukemia. Blood 123:1229-38 (2014). Human . Read more (PubMed: 24307721) »
    • Daniel JL  et al. Cbl-b is a novel physiologic regulator of glycoprotein VI-dependent platelet activation. J Biol Chem 285:17282-91 (2010). Read more (PubMed: 20400514) »

    See all 3 Publications for this product

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