重组Anti-BTK (phospho Y223)抗体[EP420Y] (ab68217)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP420Y] to BTK (phospho Y223)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BTK (phospho Y223)抗体[EP420Y]
参阅全部 BTK 一抗 -
描述
兔单克隆抗体[EP420Y] to BTK (phospho Y223) -
宿主
Rabbit -
经测试应用
适用于: WB, IP, Dot blotmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab214589) -
阳性对照
- WB: K562 and Ramos cell lysates treated with pervanadate. IP: Ramos cell lysates treated with pervanadate. Dot Blot: BTK (phospho Y223) phospho peptide.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP420Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with BTK peptide (ab214589).
For unpurified, use 1/10000 - 1/50000. |
|
IP |
1/20.
For unpurified, use 1/40. |
|
Dot blot |
Use at an assay dependent concentration.
|
说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with BTK peptide (ab214589). For unpurified, use 1/10000 - 1/50000. |
IP
1/20. For unpurified, use 1/40. |
Dot blot
Use at an assay dependent concentration. |
靶标
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功能
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. -
疾病相关
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein. -
细胞定位
Cytoplasm. Membrane. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 695 Human
- Entrez Gene: 12229 Mouse
- Entrez Gene: 367901 Rat
- Omim: 300300 Human
- SwissProt: Q06187 Human
- SwissProt: P35991 Mouse
- Unigene: 159494 Human
- Unigene: 4475 Mouse
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别名
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
see all
图片
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All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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ab68217 (purified) at 1/50 immunoprecipitating BTK (phospho Y223) in Ramos cells (Lane 1) treated with 1 mM Pervanadate for 30 min. For western blotting, a HRP-conjugated anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Dot blot analysis of BTK (phospho Y223) phospho peptide (Lane 1) and BTK phospho peptide (Lane 2) labeling BTK (phospho Y223) with ab68217 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217) at 1/10000 dilution (purified)
Lane 1 : Untreated K562 cell lysate
Lane 2 : K562 cells treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab68217 被引用在 19 文献中.
- Chen T et al. The effect of geniposide on chronic unpredictable mild stress-induced depressive mice through BTK/TLR4/NF-?B and BDNF/TrkB signaling pathways. Phytother Res 35:932-945 (2021). PubMed: 33164233
- Eisenmann ED et al. Intentional Modulation of Ibrutinib Pharmacokinetics through CYP3A Inhibition. Cancer Res Commun 1:79-89 (2021). PubMed: 34950932
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Liu S et al. The protective effect of Geniposide on diabetic cognitive impairment through BTK/TLR4/NF-?B pathway. Psychopharmacology (Berl) 237:465-477 (2020). PubMed: 31811349
- Kim E et al. The BET inhibitor GS-5829 targets chronic lymphocytic leukemia cells and their supportive microenvironment. Leukemia 34:1588-1598 (2020). PubMed: 31862959