Anti-BTK抗体[7F12H4,6G5F6] (ab54219)

概述

  • 产品名称Anti-BTK抗体[7F12H4,6G5F6]
    参阅全部 BTK 一抗
  • 描述
    小鼠单克隆抗体[7F12H4,6G5F6] to BTK
  • 经测试应用适用于: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human BTK aa 458-626.

  • 阳性对照
    • K562 cell lysate (WB), Human Lymph node tissue (IHC-P), Human Lymph follicle tissue (IHC-P), Jurkat cells (ICC/IF).

性能

应用

Our Abpromise guarantee covers the use of ab54219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 76 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • 疾病相关Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • 细胞定位Cytoplasm. Membrane. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Agammaglobulinaemia tyrosine kinase antibody
    • AGMX 1 antibody
    • AGMX1 antibody
    • AT antibody
    • ATK antibody
    • B cell progenitor kinase antibody
    • B-cell progenitor kinase antibody
    • BPK antibody
    • Bruton agammaglobulinemia tyrosine kinase antibody
    • Bruton tyrosine kinase antibody
    • Bruton’s Tyrosine Kinase antibody
    • Btk antibody
    • BTK_HUMAN antibody
    • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
    • IMD 1 antibody
    • IMD1 antibody
    • MGC126261 antibody
    • MGC126262 antibody
    • OTTHUMP00000063593 antibody
    • PSCTK 1 antibody
    • PSCTK1 antibody
    • truncated Bruton agammaglobulinemia tyrosine kinase antibody
    • Tyrosine protein kinase BTK antibody
    • Tyrosine-protein kinase BTK antibody
    • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
    • XLA antibody
    see all

Anti-BTK antibody [7F12H4,6G5F6] 图像

  • All lanes : Anti-BTK antibody [7F12H4,6G5F6] (ab54219) at 1/500 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Truncated BTK recombinant protein
    Lane 3 : K562 cell lysate


    Predicted band size : 76 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human lymph node tissue, showing cytoplasmic and membrane localization, using ab54219 (1/500 dilution) with DAB staining.
  • Immunohistochemical analysis of paraffin embedded human lymph follicle tissue, showing cytoplasmic and membrane localization, using ab54219 (1/500 dilution) with DAB staining.
  • Immunofluorescence analysis of Jurkat cells using ab54219 with FITC-IgG.
  • Overlay histogram showing Jurkat cells stained with ab54219 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54219, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-BTK antibody [7F12H4,6G5F6] (ab54219)参考文献

This product has been referenced in:
  • Landon AL  et al. MNKs act as a regulatory switch for eIF4E1 and eIF4E3 driven mRNA translation in DLBCL. Nat Commun 5:5413 (2014). WB . Read more (PubMed: 25403230) »
  • Bogusz AM  et al. Quantitative Immunofluorescence Reveals the Signature of Active B-cell Receptor Signaling in Diffuse Large B-cell Lymphoma. Clin Cancer Res 18:6122-35 (2012). WB . Read more (PubMed: 22966017) »

See all 3 Publications for this product

Product Wall

While the exact immunogen sequence is proprietary, I may confirm that the immunogen fragment is from the C-terminal.

Thank you for your reply.

I would be pleased to provide you with the Abtrial testing discount codes. In order to do so, could you please confirm which distributor you will be purchasing the antibodies from?

I look forward to receiving...

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Thank you for contacting us and your interest in our products.

The antibodies you have chosen for your ELISA should work well together. The immunogen used to raise the mouse monoclonal ab54219 does not cover the Y223 region of BTK and they sh...

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Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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The immunogen is a fragment from the C terminus.

I hope this helps. Please let me know if you have any other questions.

Thank you for contacting Abcam.

The immunogen sequence for ab54219 is:

*****

The exact epitope sequence though has not been mapped.

If there is anything else I can help you with, please let me know.

Thank you for contacting Abcam. The recombinat fragment of BTK that was used as the immunogen has the following sequence: The exact epitope site though has not been mapped. If there is anything else I can help you with,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"