Anti-BTK抗体[1A1] (ab70587)

概述

  • 产品名称Anti-BTK抗体[1A1]
    参阅全部 BTK 一抗
  • 描述
    小鼠单克隆抗体[1A1] to BTK
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Recombinant human protein purified from E.coli (His/ABD-Btk)

  • 阳性对照
    • U937, Raji and HL60 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab70587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • 疾病相关Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • 细胞定位Cytoplasm. Membrane. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Agammaglobulinaemia tyrosine kinase antibody
    • AGMX 1 antibody
    • AGMX1 antibody
    • AT antibody
    • ATK antibody
    • B cell progenitor kinase antibody
    • B-cell progenitor kinase antibody
    • BPK antibody
    • Bruton agammaglobulinemia tyrosine kinase antibody
    • Bruton tyrosine kinase antibody
    • Bruton’s Tyrosine Kinase antibody
    • Btk antibody
    • BTK_HUMAN antibody
    • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
    • IMD 1 antibody
    • IMD1 antibody
    • MGC126261 antibody
    • MGC126262 antibody
    • OTTHUMP00000063593 antibody
    • PSCTK 1 antibody
    • PSCTK1 antibody
    • truncated Bruton agammaglobulinemia tyrosine kinase antibody
    • Tyrosine protein kinase BTK antibody
    • Tyrosine-protein kinase BTK antibody
    • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
    • XLA antibody
    see all

Anti-BTK antibody [1A1] 图像

  • All lanes : Anti-BTK antibody [1A1] (ab70587) at 1/5000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : HL60 cell lysate


    Predicted band size : 76 kDa
    Observed band size : 76 kDa

Anti-BTK antibody [1A1] (ab70587)参考文献

ab70587 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. Karin is not in the office today and I am happy to help.

I can confirm that ab70587 was purified using Ammonium sulfate precipitation. This degree of purification should be fine for using it as a capture antibody in ...

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Thank you for your call earlier this week and for your patience while I have been in touch with the lab regarding ab70587. Unfortunately the concentration of this antibody is not measured. The antibody is adjusted so that optimal bands are seen in WB a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"