Anti-BTG2抗体(ab58219)

概述

  • 产品名称Anti-BTG2抗体
    参阅全部 BTG2 一抗
  • 描述
    小鼠单克隆抗体to BTG2
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: PSKGSGYRCI RINHKMDPII SRVASQIGLS QPQLHQLLPS ELTLWVDPYE VSYRIGEDGS ICVLYEEAPL AASCGLLTCK NQVLLGRSSP SKNYVMAVSS , with an additional ~26 kDa sequence. The immunogen corresponding to amino acids 59-159 of Human BTG2

性能

应用

Our Abpromise guarantee covers the use of ab58219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors (By similarity). Anti-proliferative protein. Modulates transcription regulation mediated by ESR1. Involved in neurite outgrowth.
  • 序列相似性Belongs to the BTG family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell translocation gene 2 antibody
    • BTG family member 2 antibody
    • Btg2 antibody
    • BTG2_HUMAN antibody
    • Nerve growth factor inducible anti proliferative antibody
    • NGF inducible anti proliferative protein PC3 antibody
    • NGF-inducible anti-proliferative protein PC3 antibody
    • PC3 antibody
    • Pheochromacytoma cell 3 antibody
    • Protein BTG2 antibody
    • TIS21 antibody
    see all

Anti-BTG2 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab58219 BTG2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • All lanes : Anti-BTG2 antibody (ab58219)

    Lane 1 : BTG2 transfected 293T lysate (17.4 kDa)
    Lane 2 : Non-transfected 293T lysate
  • Overlay histogram showing HEK293 cells stained with ab58219 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58219, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-BTG2 antibody (ab58219)参考文献

ab58219 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Yes, this is the protocol that was used to generate the WB image of the 17 kDa proteinseen on the online datasheet.

http://www.abcam.com/btg2-antibody-ab58219.html#BTG2-Primary-antibodies-ab58219-3.jpg
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Thank you for your inquiry.

Here is the protocol requested:

Transfected lysate (denatured):

1) Prepare transfection reagents

Reagent A: add 6 ul transfection reagent (Lipofectamine (TM) 2000) into 100ul culture mediu...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 25 µg
Specification MEF
Treatment 5 µM MG132 for 16 hrs
Gel Running Conditions Reduced Denaturing (15%)
Blocking step 2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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提交于 Jul 18 2011

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