概述

  • 产品名称Anti-BTF3抗体
    参阅全部 BTF3 一抗
  • 描述
    兔多克隆抗体to BTF3
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Zebrafish
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Saccharomyces cerevisiae, Drosophila melanogaster
  • 免疫原

    Synthetic peptide: DSLTSLRRLA EALPKQSVDG KAPLATGEDD DDEVPDLVEN FDEASKNEAN , corresponding to amino acids 155-204 of Human BTF3

  • 阳性对照
    • Jurkat cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab66940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 23, 18 kDa (predicted molecular weight: 23 , 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-BTF3 antibody 图像

  • Anti-BTF3 antibody (ab66940) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 , 18 kDa
    Observed band size : 23 and 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa (possible cross reactivity).
    Gel concentration 12%
  • IHC-P image of BTF3 (ab66940) on Testis sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 mins at 22°C.

    See Abreview

Anti-BTF3 antibody (ab66940)参考文献

This product has been referenced in:
  • Thakur D  et al. Human Beta Casein Fragment (54-59) Modulates M. bovis BCG Survival and Basic Transcription Factor 3 (BTF3) Expression in THP-1 Cell Line. PLoS One 7:e45905 (2012). WB . Read more (PubMed: 23029305) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10 % GEL, SDS)
Sample Human Cell lysate - whole cell (HEK293, HELA, HCT116)
Specification HEK293, HELA, HCT116
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Apr 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis, non-malignant)
Specification Testis, non-malignant
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novacastra pH 6
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

提交于 Oct 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"