概述

  • 产品名称Anti-BTF3抗体
    参阅全部 BTF3 一抗
  • 描述
    兔多克隆抗体to BTF3
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 35-70 of Human BTF3 (NP_001032726.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab113974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能General transcription factor. BTF3 can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
    • 序列相似性Belongs to the NAC-beta family.
      Contains 1 NAC-A/B (NAC-alpha/beta) domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Basic transcription factor 3 antibody
      • BETA NAC antibody
      • BTF 3 antibody
      • btf3 antibody
      • BTF3_HUMAN antibody
      • BTF3a antibody
      • BTF3b antibody
      • NACB antibody
      • Nascent polypeptide associated complex beta polypeptide antibody
      • RNA polymerase B transcription factor 3 antibody
      • Transcription factor BTF3 antibody
      see all

    Anti-BTF3 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and human prostate carcinoma (right) tissues labelling BTF3 with ab113974 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of BTF3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab113974 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-BTF3 antibody which recognizes a upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

      Predicted band size : 22 kDa.

    Anti-BTF3 antibody (ab113974)参考文献

    ab113974 has not yet been referenced specifically in any publications.

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