Chimpanzee, Rhesus monkey, Gorilla, Bat
Synthetic peptide corresponding to a region between amino acids 35-70 of Human BTF3 (NP_001032726.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 10 µg/mg of lysate.
Is unsuitable for WB.
General transcription factor. BTF3 can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Belongs to the NAC-beta family.
Contains 1 NAC-A/B (NAC-alpha/beta) domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Basic transcription factor 3 antibody
BETA NAC antibody
BTF 3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF3 antibody (ab113974)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and human prostate carcinoma (right) tissues labelling BTF3 with ab113974 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-BTF3 antibody (ab113974)
Detection of BTF3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab113974 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-BTF3 antibody which recognizes a upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Predicted band size : 22 kDa.
has not yet been referenced specifically in any publications.
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