Anti-BTB/POZ domain containing protein 6抗体(ab66788)

概述

  • 产品名称Anti-BTB/POZ domain containing protein 6抗体
    参阅全部 BTB/POZ domain containing protein 6 一抗
  • 描述
    兔多克隆抗体to BTB/POZ domain containing protein 6
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Cat
  • 免疫原

    A region within synthetic peptide: MFNNELMADV HFVVGPPGAT RTVPAHKYVL AVGSSVFYAM FYGDLAEVKS, corresponding to N terminal amino acids 1-50 of Human BTB/POZ domain containing protein 6

  • 阳性对照
    • Wb: HepG2 cell lysate IHC-P: Human intestinal tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能Adapter protein for the CUL3 E3 ubiquitin-protein ligase complex. Involved in late neuronal development and muscle formation.
  • 组织特异性Expressed in lens.
  • 序列相似性Contains 1 BTB (POZ) domain.
  • 细胞定位Cytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AF1 coactivator 1| antibody
    • BDPL antibody
    • BTB (POZ) domain containing 6 antibody
    • BTB domain protein BDPL antibody
    • BTB/POZ domain-containing protein 6 antibody
    • BTBD 6 antibody
    • BTBD6 antibody
    • BTBD6_HUMAN antibody
    • Glucocorticoid receptor AF 1 coactivator 1 antibody
    • Lens BTB domain protein antibody
    • OTTHUMP00000163908 antibody
    see all

Anti-BTB/POZ domain containing protein 6 antibody 图像

  • Anti-BTB/POZ domain containing protein 6 antibody (ab66788) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling BTB/POZ with ab66788 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the villi. Magnification: 400X.

Anti-BTB/POZ domain containing protein 6 antibody (ab66788)参考文献

ab66788 has not yet been referenced specifically in any publications.

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