Anti-BTBD15抗体(ab117775)

概述

  • 产品名称Anti-BTBD15抗体
    参阅全部 BTBD15 一抗
  • 描述
    兔多克隆抗体to BTBD15
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 300-350 of Human BTBD15 (BAI46608.1).

  • 阳性对照
    • Jurkat, HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab117775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能May be involved in transcriptional regulation.
  • 序列相似性Contains 1 BTB (POZ) domain.
    Contains 4 C2H2-type zinc fingers.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BTB (POZ) domain containing 15 antibody
    • BTB/POZ domain-containing protein 15 antibody
    • BTBD15 antibody
    • HSPC063 antibody
    • MGC26123 antibody
    • MGC57431 antibody
    • MGC60348 antibody
    • MGC88058 antibody
    • OTTHUMP00000230934 antibody
    • OTTHUMP00000230935 antibody
    • OTTHUMP00000230937 antibody
    • OTTHUMP00000230938 antibody
    • ZBT44_HUMAN antibody
    • Zbtb44 antibody
    • Zinc finger and BTB domain containing 44 antibody
    • Zinc finger and BTB domain-containing protein 44 antibody
    • Zinc finger protein 851 antibody
    • ZNF851 antibody
    see all

Anti-BTBD15 antibody 图像

  • All lanes : Anti-BTBD15 antibody (ab117775) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 64 kDa


    Exposure time : 30 seconds
  • Detection of BTBD15 by Western Blot of Immunprecipitate.
    ab117775 at 1µg/ml staining BTBD15 in Jurkat whole cell lysate immunoprecipitated using ab117775 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-BTBD15 antibody (ab117775)参考文献

ab117775 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 90 µg
Gel Running Conditions Reduced Denaturing (6-18% gradient gel)
Sample Human Cell lysate - whole cell (human osteosarcoma U2OS cells)
Specification human osteosarcoma U2OS cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 29 2014

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