Anti-BTBD15抗体(ab117775)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to BTBD15
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 300-350 of Human BTBD15 (BAI46608.1).

  • 阳性对照
    • Jurkat, HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab117775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能
    May be involved in transcriptional regulation.
  • 序列相似性
    Contains 1 BTB (POZ) domain.
    Contains 4 C2H2-type zinc fingers.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BTB (POZ) domain containing 15 antibody
    • BTB/POZ domain-containing protein 15 antibody
    • BTBD15 antibody
    • HSPC063 antibody
    • MGC26123 antibody
    • MGC57431 antibody
    • MGC60348 antibody
    • MGC88058 antibody
    • OTTHUMP00000230934 antibody
    • OTTHUMP00000230935 antibody
    • OTTHUMP00000230937 antibody
    • OTTHUMP00000230938 antibody
    • ZBT44_HUMAN antibody
    • Zbtb44 antibody
    • Zinc finger and BTB domain containing 44 antibody
    • Zinc finger and BTB domain-containing protein 44 antibody
    • Zinc finger protein 851 antibody
    • ZNF851 antibody
    see all

图片

  • All lanes : Anti-BTBD15 antibody (ab117775) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 64 kDa


    Exposure time : 30 seconds
  • Detection of BTBD15 by Western Blot of Immunprecipitate.
    ab117775 at 1µg/ml staining BTBD15 in Jurkat whole cell lysate immunoprecipitated using ab117775 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

文献

ab117775 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
90 µg
Gel Running Conditions
Reduced Denaturing (6-18% gradient gel)
Sample
Human Cell lysate - whole cell (human osteosarcoma U2OS cells)
Specification
human osteosarcoma U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册