Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide, corresponding to a region within amino acids 300-350 of Human BTBD15 (BAI46608.1).
Jurkat, HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
ab117775 was affinity purified using an epitope specific to BTBD15 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in transcriptional regulation.
Contains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
BTB (POZ) domain containing 15 antibody
BTB/POZ domain-containing protein 15 antibody
Western blot - Anti-BTBD15 antibody (ab117775)
All lanes : Anti-BTBD15 antibody (ab117775) at 0.1 µg/ml Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 64 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-BTBD15 antibody (ab117775)
Detection of BTBD15 by Western Blot of Immunprecipitate.
ab117775 at 1µg/ml staining BTBD15 in Jurkat whole cell lysate immunoprecipitated using ab117775 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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