The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
相关性BTBD10 appears to behave as a suppressor of cell death, which includes neuronal cell death related to amyotrophic lateral sclerosis. It may also act as an enhancer of cell growth via its positive regulation of Akt phosphorylation.