概述

  • 产品名称Anti-BST2抗体
    参阅全部 BST2 一抗
  • 描述
    小鼠多克隆抗体to BST2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human BST2 protein (NP_004326.1).

  • 阳性对照
    • Human spleen tissue lysate, Jurkat cell lysate and BST2 transfected 293T cell lysate Human kidney tissue HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab88523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
  • 组织特异性Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
  • 序列相似性Belongs to the tetherin family.
  • 结构域The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
  • 翻译后修饰Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
  • 细胞定位Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bone marrow stromal antigen 2 antibody
    • Bone marrow stromal cell antigen 2 antibody
    • Bone marrow stromal cell antigen antibody
    • BST 2 antibody
    • BST-2 antibody
    • BST2 antibody
    • BST2_HUMAN antibody
    • CD 317 antibody
    • CD317 antibody
    • CD317 antigen antibody
    • HM1.24 antigen antibody
    • NPC A 7 antibody
    • Tetherin antibody
    see all

Anti-BST2 antibody 图像

  • Anti-BST2 antibody (ab88523) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Anti-BST2 antibody (ab88523) at 1 µg/ml + jurkat cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab88523, at 3 µg/ml, staining BST2 in formalin fixed, paraffin embedded human kidney tissue sections by Immunoperoxidase/Immunohistochemistry.
  • ab88523 at 10 µg/ml staining BST2 in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.

Anti-BST2 antibody (ab88523)参考文献

This product has been referenced in:
  • Nityanandam R & Serra-Moreno R BCA2/Rabring7 targets HIV-1 Gag for lysosomal degradation in a tetherin-independent manner. PLoS Pathog 10:e1004151 (2014). WB . Read more (PubMed: 24852021) »
  • Serra-Moreno R  et al. Tetherin/BST-2 antagonism by Nef depends on a direct physical interaction between Nef and tetherin, and on clathrin-mediated endocytosis. PLoS Pathog 9:e1003487 (2013). WB . Read more (PubMed: 23853598) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry.

Unfortunately we are not aware of any publication using the ab88523. This does not mean that this antibody has not been used in a publication, it just means that we do not know about it.

I can however co...

Read More

Thank you for your reply.
I'm sending a free of charge vial of ab14694 on the order ***, which should arrive on Monday. Please keep me updated about the performance of this replacement antibody.
I look forward to hearing from you. Please let ...

Read More

Thank you for your reply and for providing so many details about the protocol. This information will be helpful for our investigation of this antibody.
I've found an order going to ***. Can you confirm that this is your order? I can send the repla...

Read More

According to our records, ab88523 was proving difficult to use in Western blot and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to help us better unders...

Read More

Thank you for contacting us and letting us know about the trouble with this antibody.
I do have a couple of additional questions, so that I can better understand the situation:
1) Do you know your order number or lot number?
2) How has t...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"