概述

  • 产品名称
  • 描述
    兔多克隆抗体to BST2
  • 经测试应用
    适用于: Dot blot, ELISA, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide (Human) (Position 121-140 on human BST2).

  • 阳性对照
    • Rat neonatal brain.

性能

应用

Our Abpromise guarantee covers the use of ab14694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot 1/2000.
ELISA 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

靶标

  • 功能
    May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
  • 组织特异性
    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
  • 序列相似性
    Belongs to the tetherin family.
  • 结构域
    The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
  • 翻译后修饰
    Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
  • 细胞定位
    Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bone marrow stromal antigen 2 antibody
    • Bone marrow stromal cell antigen 2 antibody
    • Bone marrow stromal cell antigen antibody
    • BST 2 antibody
    • BST-2 antibody
    • BST2 antibody
    • BST2_HUMAN antibody
    • CD 317 antibody
    • CD317 antibody
    • CD317 antigen antibody
    • HM1.24 antigen antibody
    • NPC A 7 antibody
    • Tetherin antibody
    see all

实验方案

文献

This product has been referenced in:
  • Nishitsuji H  et al. ATP1B3 Protein Modulates the Restriction of HIV-1 Production and Nuclear Factor ? Light Chain Enhancer of Activated B Cells (NF-?B) Activation by BST-2. J Biol Chem 291:4754-62 (2016). Read more (PubMed: 26694617) »
  • Barrett BS  et al. A single nucleotide polymorphism in tetherin promotes retrovirus restriction in vivo. PLoS Pathog 8:e1002596 (2012). Read more (PubMed: 22457621) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for your enquiry. I can confirm that this product, ab14694 recognizes both the precursor protein of around 21kDa and the post translationally modified form of higher molecular weights. I hope this information will be helpful. If there...

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Thank you for your enquiry. Ab14694 cross-reacts with human and rat BST2, and cross-reacts weakly with mouse. Rat neonatal brain or human recombinant protein are recommended as positive controls. If you have any additional questions, please contact...

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Thank you for your enquiry. To answer your question. Yes, the antibody does contain 0.001% sodium azide with other preservatives. The antibody stabilization buffer also contains stabilizing proteins beside IgGs. I hope this information helps. P...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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