概述

  • 产品名称Anti-BSD抗体
  • 描述
    兔多克隆抗体to BSD
  • 特异性The antibody detects a 13 kDa protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    not applicable
  • 免疫原

    Synthetic peptide conjugated to KLH corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was affinity purified using the N-terminal Bsd peptide (without carrier).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 13 kDa.
ELISA 1/4000.
ICC/IF 1/100.

靶标

  • 相关性Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week.
  • 别名
    • Blasticidin-S deaminase antibody
    • BSD antibody

Anti-BSD antibody 图像

  • All lanes : Anti-BSD antibody (ab38307)

    Lane 1 : 293 cells mock transfected
    Lane 2 : 293 cells transiently transfected with pLenti6/TR lentiviral vector
    Lane 3 : 293 cells transiently transfected with pLenti6/TR lentiviral vector


    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    Each lane was loaded with 10-20µg total protein.
  • ab38307, staining BSD in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min. The primary was used at 1/100 and then an appropriate secondary antibody conjugated to Texas Red was used.

Anti-BSD antibody (ab38307)参考文献

This product has been referenced in:
  • Jammart B  et al. Very-low-density lipoprotein (VLDL)-producing and hepatitis C virus-replicating HepG2 cells secrete no more lipoviroparticles than VLDL-deficient Huh7.5 cells. J Virol 87:5065-80 (2013). Read more (PubMed: 23427158) »
  • de Wispelaere M & Yang PL Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly. J Virol 86:7072-83 (2012). Read more (PubMed: 22532681) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Fibroblasts transduced with BSD-resistance gene)
Specification Fibroblasts transduced with BSD-resistance gene
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citrate (pH 6.)
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Oct 08 2012

DISCOUNT CODE: ***
Expiration date: October 5, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab38307 in IHC-Pand include this code in the “Additi...

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Thanks for your call today and for your interest in ab38307. Please see below for your code to test this antibody in paraffin embedded tissue staining (IHC-P) and let me know if you have any questions.

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Thank you for contacting us. I am sorry to hear you have been experiencing problems with ab38307. The customer has made many changes to optimize and it is disappointing that the antibody is still not working. I would be happy to refund or repla...

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Thank you for your inquiry. The cells in the ICC/IF image for ab38307 were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min, then the labeling was performed ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"