概述

  • 产品名称
  • 描述
    兔多克隆抗体to BRP44
  • 特异性
    ab111380 detects endogenous levels of total BRP44 protein.
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal region of Human BRP44.

  • 阳性对照
    • Human lung carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride
    Note: without Mg2+ and Ca2+
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab111380 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-BRP44 antibody 图像

  • ab111380 at 1/50 dilution staining BRP44 in paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-BRP44 antibody (ab111380)参考文献

This product has been referenced in:
  • Li Y  et al. Mitochondrial pyruvate carrier function is negatively linked to Warburg phenotype in vitro and malignant features in esophageal squamous cell carcinomas. Oncotarget 8:1058-1073 (2017). WB . Read more (PubMed: 27911865) »
  • Li Y  et al. PDHA1 gene knockout in prostate cancer cells results in metabolic reprogramming towards greater glutamine dependence. Oncotarget 7:53837-53852 (2016). Read more (PubMed: 27462778) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Leukemia cell lines)
Gel Running Conditions
Reduced Denaturing (4-20% and 18%)
Loading amount
40 µg
Specification
Leukemia cell lines
Blocking step
Licor:PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 04 2016

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Lane 1 U2OS, Lane 2 MIN6)
Specification
Lane 1 U2OS, Lane 2 MIN6
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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