概述

  • 产品名称
  • 描述
    兔多克隆抗体to BROX
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: LGNLVKNTLE KCQRENGFIY FQKIPTEAPQ LELKANYGLV EPIPFEFPPT SVQWTPETLA AFDLTKRPKD DSTKPKPEEE VKPVKEPDIK PQKDTGCYIS, corresponding to amino acids of Human BROX

  • 阳性对照
    • Human urinary bladder tissue; RT 4, U 251 MG, Human liver and tonsil lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 序列相似性
    Belongs to the BROX family.
    Contains 1 BRO1 domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRO1 domain and CAAX motif containing antibody
    • BRO1 domain containing protein antibody
    • BRO1 domain- and CAAX motif-containing protein antibody
    • BRO1 domain-containing protein BROX antibody
    • BROFTI antibody
    • Brox antibody
    • BROX_HUMAN antibody
    • C1orf58 antibody
    • FLJ32421 antibody
    • hCG_25336 antibody
    • RP11-452F19.1 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122174 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122174, at 1/500 dilution, staining BROX in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
  • All lanes : Anti-BROX antibody (ab122174) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

文献

ab122174 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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