antigen sequence: LGNLVKNTLE KCQRENGFIY FQKIPTEAPQ LELKANYGLV EPIPFEFPPT SVQWTPETLA AFDLTKRPKD DSTKPKPEEE VKPVKEPDIK PQKDTGCYIS, corresponding to amino acids of Human BROX
Human urinary bladder tissue; RT 4, U 251 MG, Human liver and tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the BROX family.
Contains 1 BRO1 domain.
Information by UniProt
BRO1 domain and CAAX motif containing antibody
BRO1 domain containing protein antibody
BRO1 domain- and CAAX motif-containing protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-BROX antibody (ab122174)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122174 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BROX antibody (ab122174)
ab122174, at 1/500 dilution, staining BROX in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
Western blot - Anti-BROX antibody (ab122174)
All lanes : Anti-BROX antibody (ab122174) at 1/250 dilution Lane 1 : RT 4 lysate Lane 2 : U 251 MG lysate Lane 3 : Human plasma lysate Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"