The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain.
Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) [MIM:304400]. A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness.
Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.