概述

  • 产品名称Anti-BRN3B / POU4F2抗体
    参阅全部 BRN3B / POU4F2 一抗
  • 描述
    兔多克隆抗体to BRN3B / POU4F2
  • 特异性This antibody reacts with BRN3B / POU4F2.
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human BRN3B/ POU4F2 aa 1-50 (N terminal).
    Sequence:

    MMMMSLNSKQAFSMPHGGSLHVEPKYSALHSTSPGSSAPIAPSASSPSSS

  • 阳性对照
    • WB: HepG2 cell lysate. IF/ICC: SKNSH cell line

性能

应用

Our Abpromise guarantee covers the use of ab56026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • 组织特异性Brain. Seems to be specific to the retina.
  • 序列相似性Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 细胞定位Nucleus speckle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brain specific homeobox/POU domain protein 3B antibody
    • Brain-3B antibody
    • Brain-specific homeobox/POU domain protein 3B antibody
    • Brn-3B antibody
    • BRN3.2 antibody
    • BRN3B antibody
    • Brn3b POU domain transcription factor antibody
    • class 4 antibody
    • PO4F2_HUMAN antibody
    • POU class 4 homeobox 2 antibody
    • POU domain antibody
    • POU domain class 4 transcription factor 2 antibody
    • POU domain protein antibody
    • POU4F2 antibody
    • transcription factor 2 antibody
    see all

Anti-BRN3B / POU4F2 antibody 图像

  • ICC/IF image of ab56026 stained Malme-3M cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56026, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-BRN3B / POU4F2 antibody (ab56026) at 0.2 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-BRN3B / POU4F2 antibody (ab56026)参考文献

This product has been referenced in:
  • Tucker BA  et al. Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation. Stem Cells Transl Med 2:16-24 (2013). Read more (PubMed: 23283489) »

See 1 Publication for this product

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To our knowledge, we do not have an alternative antibody which has been tested in IHC so far, although we have two mor...

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Thank you for your telephone enquiry yesterday afternoon.

I am sorry to confirm that ab32264 BRN3B / POU4F2 antibodyis not longer available.

As discussed on the telephone, we do have an alternative, ab56026. I can recommend to rev...

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