概述

  • 产品名称Anti-BRMS1抗体
    参阅全部 BRMS1 一抗
  • 描述
    兔多克隆抗体to BRMS1
  • 特异性ab65244 detects endogenous levels of total BRMS1 protein.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from N terminal of human BRMS1.

  • 阳性对照
    • 293 cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 22076152
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

靶标

  • 功能Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
  • 组织特异性Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
  • 序列相似性Belongs to the BRMS1 family.
  • 细胞定位Nucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AV003220 antibody
    • AW554636 antibody
    • Breast cancer metastasis suppressor 1 antibody
    • Breast cancer metastasis-suppressor 1 antibody
    • BRMS1 antibody
    • BRMS1_HUMAN antibody
    • DKFZp564A063 antibody
    • MGC95128 antibody
    see all

Anti-BRMS1 antibody 图像

  • All lanes : Anti-BRMS1 antibody (ab65244) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide


    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • ab65244 staining BRMS1 in Human breast cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was blocked with 10% goat serum for 60 minutes; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in diluent) overnight at 4°C, incubated with HRP-conjugated secondary antibody, before being stained with DAB.

Anti-BRMS1 antibody (ab65244)参考文献

This product has been referenced in:
  • Al-Alwan M  et al. Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules. PLoS One 6:e27339 (2011). IHC-P ; Human . Read more (PubMed: 22076152) »

See 1 Publication for this product

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