概述

  • 产品名称Anti-Brk抗体
    参阅全部 Brk 一抗
  • 描述
    兔多克隆抗体to Brk
  • 特异性ab65221 detects endogenous levels of total Brk protein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from N terminus of human Brk

  • 阳性对照
    • LOVO cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab65221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

靶标

  • 功能Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
  • 组织特异性Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
  • 细胞定位Cytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Breast tumor kinase antibody
    • Breast tumour kinase antibody
    • Protein tyrosine kinase 6 antibody
    • Protein-tyrosine kinase 6 antibody
    • Ptk6 antibody
    • PTK6_HUMAN antibody
    • Tyrosine protein kinase BRK antibody
    • Tyrosine-protein kinase BRK antibody
    see all

Anti-Brk antibody 图像

  • All lanes : Anti-Brk antibody (ab65221) at 1/500 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa

Anti-Brk antibody (ab65221)参考文献

ab65221 has not yet been referenced specifically in any publications.

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