概述

  • 产品名称Anti-BRIT1抗体
    参阅全部 BRIT1 一抗
  • 描述
    兔多克隆抗体to BRIT1
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen, corresponding to amino acids 555-701 of Human BRIT1 (Q8NEM0).

  • 阳性对照
    • Human cerebral cortex RT-4 cell lysate U-251 MG cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab121277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
  • 组织特异性Expressed in fetal brain, liver and kidney.
  • 疾病相关Defects in MCPH1 are the cause of microcephaly primary type 1 (MCPH1) [MIM:251200]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait.
  • 序列相似性Contains 3 BRCT domains.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRCT repeat inhibitor of TERT expression 1 antibody
    • BRIT 1 antibody
    • FLJ12847 antibody
    • Hypothetical protein FLJ12847 antibody
    • MCPH 1 antibody
    • MCPH1 antibody
    • MCPH1_HUMAN antibody
    • MCT antibody
    • Microcephalin 1 antibody
    • Microcephalin antibody
    • Microcephaly primary autosomal recessive 1 antibody
    see all

Anti-BRIT1 antibody 图像

  • All lanes : Anti-BRIT1 antibody (ab121277) at 1/250 dilution

    Lane 1 : Markers (kDa)
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human plasma
    Lane 5 : Liver tissue lysate
    Lane 6 : Tonsil tissue lysate

    Secondary
    HPR conjugated anti-rabbit antibody at 1/3000 dilution
    Developed using the ECL technique
  • ab121277, at 1/75, staining BRIT1 in neuronal cells of paraffin embedded Human cerebral cortex by Immunohistochemistry.
  • Immunohistochemical staining of Human testis shows strong positivity in a subset of cells in seminiferus ducts using ab121277

Anti-BRIT1 antibody (ab121277)参考文献

This product has been referenced in:
  • Ge C  et al. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS One 10:e0144957 (2015). Read more (PubMed: 26683461) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (U2OS and RPE)
Total protein in input 3.5 µg
Specification U2OS and RPE
Immuno-precipitation step Protein A
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提交于 Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"