概述

  • 产品名称Anti-Brevican抗体
    参阅全部 Brevican 一抗
  • 描述
    兔多克隆抗体to Brevican
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 83-262 of Human Brevican (AAH09117).

  • 阳性对照
    • Human breast carcinoma tissue; MCF7 whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab111719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C-terminal epitope EAMESE.
  • 细胞定位Isoform 1 is secreted, extracellular space, extracellular matrix; Isoform 2 is found on the membrane; Lipid-anchor, GPI-anchor.
  • 数据库链接
  • 别名
    • BCAN antibody
    • BEHAB antibody
    • Brain enriched hyaluronan binding protein antibody
    • Brevican core protein antibody
    • Brevican core protein isoform 1 antibody
    • Brevican core protein isoform 2 antibody
    • Brevican proteoglycan antibody
    • Chondroitin sulfate proteoglycan 7 antibody
    • Chondroitin sulfate proteoglycan BEHAB antibody
    • CSPG7 antibody
    • MGC13038 antibody
    see all

Anti-Brevican antibody 图像

  • Anti-Brevican antibody (ab111719) at 1/500 dilution + MCF-7 whole cell lysate at 30 µg

    Predicted band size : 99 kDa
  • ab111719 at 1/500 dilution staining Brevican in Formalin-fixed, paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

Anti-Brevican antibody (ab111719)参考文献

This product has been referenced in:
  • Orlando C  et al. Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner. J Neurosci 32:18009-17, 18017a (2012). ICC/IF ; Mouse . Read more (PubMed: 23238717) »

See 1 Publication for this product

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Thank you for submitting an Abreview for ab111719. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (human astrocytes)
Specification human astrocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Oct 05 2012

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Do you happen to know which region of Brevican is membrane bound?The immunogen for ab111719 falls within the region betweenamino acids 83 and 262 of human Brevican. According to the SwissProt entry for this pro...

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I am very pleased to hear you would like to accept our offer and test ab111719 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer,...

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