Anti-BrdU抗体- Proliferation Marker (ab1893)

概述

  • 产品名称
    Anti-BrdU抗体- Proliferation Marker
    参阅全部 BrdU 一抗
  • 描述
    羊多克隆抗体to BrdU - Proliferation Marker
  • 经测试应用
    适用于: IHC-FrFl, IHC-P, IHC-Fr, ICC/IF, ELISA, WB, IHC-FoFrmore details
  • 免疫原

    Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)

性能

应用

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21118958
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19332057

靶标

  • 相关性
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 细胞定位
    Nuclear
  • 别名
    • Bromodeoxyuridine antibody
    • BUdr antibody

图片

  • ab1893 staining BrdU in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.

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  • ab1893 staining BrdU in Ramos cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Kim SH  et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). ICC/IF ; Human . Read more (PubMed: 27926513) »
  • Xie Q  et al. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription. Nucleic Acids Res 45:2472-2489 (2017). Read more (PubMed: 27924000) »

See all 76 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ramos cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization
No
Specification
Ramos cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 25 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cultured Cells (COS7)
Specification
COS7
Permeabilization
Yes - 0.5% TritonX100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 27 2014

Thank you for contacting us.

Unfortunately we do not have any cross-reactivity data against FDU either.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your telephone call yesterday afternoon.

I have received some further information regarding the protocol for use with ab1893 BrdU antibody.

There are two protocols we are aware of that have been used with this antibo...

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Thank you for contacting us. Cultured cells should be pulsed with 10uM BrdU for 1 hour - overnight. I hope this helps, please let me know if you need any additional information or assistance.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (colon cancer)
Specification
colon cancer
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 07 2012

T
Thank you for your patience in this matter.



I have been trying to determine if any of our BrdU antibodies are only specific to BrdU or not and it seems that most of them have some sort of cross reactivity with either ErdU or ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Paraformaldehyde fixed mouse brain sections)
Specification
Paraformaldehyde fixed mouse brain sections
Fixative
No
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 19 2012

Thank you for contacting us.

We have checked the instock vials and can confirm that the liquid is frozen at -20C. You may need to check the freezer temperature because the antibody buffer should get frozen at -20C.

There is also n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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