概述

  • 产品名称Anti-BrdU抗体[BU6-4]
    参阅全部 BrdU 一抗
  • 描述
    小鼠单克隆抗体[BU6-4] to BrdU
  • 特异性This antibody specifically reacts with bromodeoxyuridine.
  • 经测试应用适用于: ELISA, Flow Cyt, WBmore details
  • 免疫原

    Bromodeoxyuridine-BSA conjugate.

性能

应用

Our Abpromise guarantee covers the use of ab12219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Use under non reducing condition.

靶标

  • 相关性The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 细胞定位Nuclear
  • 别名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [BU6-4] (ab12219)参考文献

This product has been referenced in:
  • Irwin RW  et al. Allopregnanolone preclinical acute pharmacokinetic and pharmacodynamic studies to predict tolerability and efficacy for Alzheimer's disease. PLoS One 10:e0128313 (2015). Read more (PubMed: 26039057) »
  • Singh C  et al. Allopregnanolone restores hippocampal-dependent learning and memory and neural progenitor survival in aging 3xTgAD and nonTg mice. Neurobiol Aging 33:1493-506 (2012). Flow Cyt . Read more (PubMed: 21803451) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"