HRP Anti-BrdU抗体[BU1/75 (ICR1)] (ab220507)
Key features and details
- HRP Rat monoclonal [BU1/75 (ICR1)] to BrdU
- Suitable for: IHC-P
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
HRP Anti-BrdU抗体[BU1/75 (ICR1)]
参阅全部 BrdU 一抗 -
描述
HRP大鼠单克隆抗体[BU1/75 (ICR1)] to BrdU -
宿主
Rat -
偶联物
HRP -
特异性
This antibody reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not react with thymidine. It has been reported in the literature that this antibody clone cross-reacts with Edu (PMID: 23272138) and some customers reported that it cross reacts with IdU.
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
The details of the immunogen for this antibody are not available.
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阳性对照
- IHC-P: BrdU treated rat skin tissue sections
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常规说明
The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available in "Neuroscience protocols" on our "Protocol and troubleshooting tips" webpage.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
BU1/75 (ICR1) -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220074)
- Alexa Fluor® 647 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220075)
- Alexa Fluor® 594 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220076)
- Alexa Fluor® 555 Anti-BrdU antibody [BU1/75 (ICR1)] (ab221240)
- Anti-BrdU antibody [BU1/75 (ICR1)] - BSA and Azide free (ab264079)
- Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
细胞定位
Nuclear -
别名
- Bromodeoxyuridine antibody
- BUdr antibody
图片
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IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded BrdU treated rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Negative IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded normal rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab220507 被引用在 1 文献中.
- Fathi I et al. Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis. Front Bioeng Biotechnol 9:756755 (2021). PubMed: 34746108