Anti-BrdU抗体[BU1/75 (ICR1)] (FITC) (ab74545)


  • 产品名称
    Anti-BrdU抗体[BU1/75 (ICR1)] (FITC)
    参阅全部 BrdU 一抗
  • 描述
    大鼠单克隆抗体[BU1/75 (ICR1)] to BrdU (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details
  • 种属反应性
    Not applicable.
  • 免疫原

    The details for the immunogen of this antibody are not available.

  • 常规说明

    This product will be discontinued shortly. We recommend the following product as an alternative:
    Anti-BrdU antibody (Alexa Fluor® 488) [BU1/75 (ICR1)] (ab220074)



Our Abpromise guarantee covers the use of ab74545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/1 - 1/20.

Use 20ul of the suggested working dilution to label 106 cells in 100ul.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 21527773
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • 相关性
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 细胞定位
  • 别名
    • Bromodeoxyuridine antibody
    • BUdr antibody


  • Detection of BrdU in cells using ab74545 counterstained with propidium iodide.


This product has been referenced in:
  • Jia HY  et al. Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions. Int J Mol Med 37:359-68 (2016). IHC ; Mouse . Read more (PubMed: 26707630) »
  • Alimbetov D  et al. Suppression of the senescence-associated secretory phenotype (SASP) in human fibroblasts using small molecule inhibitors of p38 MAP kinase and MK2. Biogerontology 17:305-15 (2016). Read more (PubMed: 26400758) »

See all 10 Publications for this product


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Standard IHC-P protocols arefollowedfor this clone. However, denaturation of the ...

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