Anti-BrdU抗体[BU1/75 (ICR1)] (Biotin) (ab74547)

概述

  • 产品名称Anti-BrdU抗体[BU1/75 (ICR1)] (Biotin)
    参阅全部 BrdU 一抗
  • 描述
    大鼠单克隆抗体[BU1/75 (ICR1)] to BrdU (Biotin)
  • 偶联物Biotin
  • 特异性This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    Not applicable.
  • 免疫原

    The details for the immunogen of this antibody are not available.

性能

应用

Our Abpromise guarantee covers the use of ab74547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. Use 20ul of the suggested working dilution to label 106 cells in 100ul.



ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 细胞定位Nuclear
  • 别名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [BU1/75 (ICR1)] (Biotin) 图像

  • ab74547 staining BrdU (red) in murine E10.5 ventral spinal cords by Immunocytochemistry/ Immunofluorescence.

Anti-BrdU antibody [BU1/75 (ICR1)] (Biotin) (ab74547)参考文献

This product has been referenced in:
  • Perez-Ruiz A  et al. beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci 121:1373-82 (2008). ICC/IF ; Mouse . Read more (PubMed: 18397993) »

See 1 Publication for this product

Product Wall

Thank you for contacting us today and your interest in our products. Please find the prodotcol used with the Anti-BrdU antibody [BU1/75 (ICR1)] (Biotin) (ab74547) to perform flow cytomerty experiments:Prepare the following solutions before proceeding:<...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"