概述

  • 产品名称Anti-BrdU抗体[BRD.3], prediluted
    参阅全部 BrdU 一抗
  • 描述
    小鼠单克隆抗体[BRD.3] to BrdU, prediluted
  • 特异性Reacts with BrdU in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU.
  • 经测试应用适用于: Flow Cyt, IHC-Pmore details
  • 种属反应性
    Not applicable
  • 免疫原

    Chemical/ Small Molecule corresponding to BrdU conjugated to Bovine Serum Albumin (BSA).

  • 阳性对照
    • Cultured cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

性能

应用

Our Abpromise guarantee covers the use of ab74443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 细胞定位Nuclear
  • 别名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [BRD.3], prediluted 图像

  • Nuclear staining of crypt epithelial cells in formalin-fixed, paraffin-embedded, BrdU-labeled mouse small intestine using ab74443 with a peroxidase-conjugated secodary antibody and a DAB chromogen.

Anti-BrdU antibody [BRD.3], prediluted (ab74443)参考文献

This product has been referenced in:
  • Tsutsui KM  et al. Nuclear protein LEDGF/p75 recognizes supercoiled DNA by a novel DNA-binding domain. Nucleic Acids Res 39:5067-81 (2011). Read more (PubMed: 21345933) »

See 1 Publication for this product

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