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Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)
Our Abpromise guarantee covers the use of ab2284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution. dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte.|
|IHC-FoFr||1/2000. 1/2000 (see Abreview).|
ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.
ab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ® 488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.
ab2284 staining BrdU in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 4°C. A Streptavidin Alexa Fluor® 488-conjugated Goat polyclonal (1/500) was used as the secondary antibody.