Anti-BrdU抗体[2B1] (ab115874)

概述

  • 产品名称Anti-BrdU抗体[2B1]
    参阅全部 BrdU 一抗
  • 描述
    小鼠单克隆抗体[2B1] to BrdU
  • 经测试应用适用于: ICC/IF, IHC-P, Flow Cytmore details
  • 免疫原

    Chemical/ Small Molecule BrdU conjugated to Ovalbumin.

  • 阳性对照
    • Mouse testis tissue and Raji cells treated with BrdU.

性能

应用

Our Abpromise guarantee covers the use of ab115874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 细胞定位Nuclear
  • 别名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [2B1] 图像

  • ab115874 staining BrdU in Mouse testis tissue, prolifierating cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with bouin, permeabilized with Tween and blocked with 20% Goat serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab115874 at 10µg/ml staining BrdU in Paraffin-embedded Mouse Testis tissue by Immunohistochemistry showing incorporation of BrdU.
  • ab115874 at 10µg/ml staining BrdU in 106 BrdU treated Raji cells by Flow cytometry (shaded Histogram).

Anti-BrdU antibody [2B1] (ab115874)参考文献

ab115874 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Sample Mouse Cell (Testis tissue, proliferating cells)
Specification Testis tissue, proliferating cells
Permeabilization Yes - TWEEN
Fixative Bouin
Username

Frau Dr. Lia Schindewolffs

Verified customer

提交于 Aug 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"