概述

  • 产品名称
  • 描述
    兔多克隆抗体to BRD8
  • 特异性
    Specific to p120
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant
  • 免疫原

    This information is considered to be commercially sensitive.

  • 阳性对照
    • Nuclear extract from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab17969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).
IP Use at 2-8 µg/mg of lysate.

靶标

  • 相关性
    BRD8 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. It interacts with MYC and adenovirus E1A protein.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • Bromodomain containing 8 antibody
    • p120 antibody
    • Skeletal muscle abundant protein antibody
    • Skeletal muscle abundant protein 2 antibody
    • SMAP antibody
    • SMAP2 antibody
    • Thyroid hormone receptor coactivating protein 120kDa antibody
    • Thyroid hormone receptor coactivating protein antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 with ab17969 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 138 kDa
    Detection of human BRD8 by Western Blot. Sample is nuclear
    extract (NE; 5, 15, and 50mcg) from HeLa cells.
  • ICC/IF image of ab17969 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Couture JP  et al. The p400/Brd8 chromatin remodeling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPAR? target genes. Endocrinology 153:5796-808 (2012). Read more (PubMed: 23064015) »
  • Yamaguchi K  et al. C20orf20 (MRG-binding protein) as a potential therapeutic target for colorectal cancer. Br J Cancer 102:325-31 (2010). WB, IP ; Human . Read more (PubMed: 20051959) »

See all 2 Publications for this product

客户评价及客户问答



There has been a revision in the entry for this target on the swissprot home page.

The epitope recognized by ab17969 is found within isoform 2 according to the current version of Uniprot entry Q9H0E9.

http://www.uniprot.or...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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