概述

  • 产品名称Anti-BRD8抗体
    参阅全部 BRD8 一抗
  • 描述
    兔多克隆抗体to BRD8
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide:

    LCLASS VMRSGDQNWV SVSRAIKPFA EPGRPPDWFSQKHCASQYSE LLETT

    , corresponding to amino acids 25-75 of Human BRD8.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab17968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Predicted molecular weight: 120 kDa.
IP Use 2-8µg for 108 cells.

靶标

  • 相关性BRD8 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. It interacts with MYC and adenovirus E1A protein.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
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Anti-BRD8 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 with ab17968 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 120 kDa

    Ab17968 at the concentrations stated, staining human BRD8 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.

     

    Ab17968 at the concentrations stated, staining human BRD8 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.

Anti-BRD8 antibody (ab17968)参考文献

This product has been referenced in:
  • Kim J  et al. A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs. Cell 143:313-24 (2010). WB ; Mouse . Read more (PubMed: 20946988) »

See 1 Publication for this product

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