Anti-Brd4抗体(ab84776)

概述

  • 产品名称Anti-Brd4抗体
    参阅全部 Brd4 一抗
  • 描述
    兔多克隆抗体to Brd4
  • 经测试应用适用于: IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1312 and 1362 of human (NP_490597.1).

  • 阳性对照
    • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Placenta, Prostate Carcinoma Mouse controls: Colon Carcinoma CT26, Renal Cell Carcinoma, Squamous Cell Carcinoma
  • 常规说明

    Concentration is optimized for IHC and not determined

性能

应用

Our Abpromise guarantee covers the use of ab84776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 1 µg/mg of lysate.
IHC-P 1/100 - 1/500.

靶标

  • 功能Plays a role in a process governing chromosomal dynamics during mitosis.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
  • 序列相似性Contains 2 bromo domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brd4 antibody
    • BRD4-NUT FUSION antibody
    • BRD4-NUT fusion oncoprotein antibody
    • BRD4_HUMAN antibody
    • Bromodomain containing 4 antibody
    • bromodomain containing protein 4 antibody
    • Bromodomain-containing protein 4 antibody
    • CAP antibody
    • chromosome associated protein antibody
    • HUNK1 antibody
    • HUNKI antibody
    • MCAP antibody
    • Mitotic chromosome-associated protein antibody
    • Protein HUNK1 antibody
    see all

Anti-Brd4 antibody 图像

  • ab84776 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/mg lysate in 50mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 anti-mouse HRP (1/10000) was used to confirm successful immunoprecipation.

     

    See Abreview

  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human colon carcinoma using a 1/500 dilution of ab84776. Detection: DAB staining
  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/100 dilution of ab84776. Detection: DAB staining

Anti-Brd4 antibody (ab84776)参考文献

ab84776 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg
Username

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Verified customer

提交于 May 07 2014

The concentration of the Brd4 antibody ab84776 is 0.1mg/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"