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Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human Brd4.
(Peptide available as ab85811.)
"Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help."
Our Abpromise guarantee covers the use of ab75898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at 1 µg/mg of lysate.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Brd4 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Hu brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75898 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"