概述

  • 产品名称Anti-BRD3抗体
    参阅全部 BRD3 一抗
  • 描述
    兔多克隆抗体to BRD3
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the C-terminal region of human BRD3 conjugated to KLH

  • 阳性对照
    • Mouse brain tissue and Saos-2 cell lysates and human breast carcinoma tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab71815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/25 - 1/100.
WB 1/100 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).

靶标

  • 组织特异性Ubiquitous.
  • 疾病相关Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • 序列相似性Contains 2 bromo domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRD 3 antibody
    • Brd3 antibody
    • BRD3_HUMAN antibody
    • Bromodomain containing 3 antibody
    • Bromodomain containing protein 3 antibody
    • Bromodomain-containing protein 3 antibody
    • KIAA0043 antibody
    • Open reading frame X antibody
    • ORFX antibody
    • RING3 like gene antibody
    • RING3-like protein antibody
    • RING3L antibody
    see all

Anti-BRD3 antibody 图像

  • All lanes : Anti-BRD3 antibody (ab71815) at 1/1000 dilution

    Lane 1 : mouse brain tissue lysate
    Lane 2 : Saos-2 cell lysate

    Lysates/proteins at 12.5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling BRD3 C-terminal with ab71815 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Anti-BRD3 antibody (ab71815)参考文献

ab71815 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Embryo)
Specification Embryo
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Username

Mr. Esteban Contreras Sepúlveda

Verified customer

提交于 Feb 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"