概述

  • 产品名称
  • 描述
    兔多克隆抗体to BRD3
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the C-terminal region of human BRD3 conjugated to KLH

  • 阳性对照
    • Mouse brain tissue and Saos-2 cell lysates and human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab71815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/25 - 1/100.
WB 1/100 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).

靶标

  • 组织特异性
    Ubiquitous.
  • 疾病相关
    Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • 序列相似性
    Contains 2 bromo domains.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRD 3 antibody
    • Brd3 antibody
    • BRD3_HUMAN antibody
    • Bromodomain containing 3 antibody
    • Bromodomain containing protein 3 antibody
    • Bromodomain-containing protein 3 antibody
    • KIAA0043 antibody
    • Open reading frame X antibody
    • ORFX antibody
    • RING3 like gene antibody
    • RING3-like protein antibody
    • RING3L antibody
    see all

图片

  • All lanes : Anti-BRD3 antibody (ab71815) at 1/1000 dilution

    Lane 1 : mouse brain tissue lysate
    Lane 2 : Saos-2 cell lysate

    Lysates/proteins at 12.5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling BRD3 C-terminal with ab71815 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

文献

ab71815 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Embryo)
Specification
Embryo
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Username

Mr. Esteban Contreras Sepúlveda

Verified customer

提交于 Feb 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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