Anti-BRD3抗体[2088C3a] (ab50818)

概述

  • 产品名称Anti-BRD3抗体[2088C3a]
    参阅全部 BRD3 一抗
  • 描述
    小鼠单克隆抗体[2088C3a] to BRD3
  • 经测试应用适用于: Flow Cyt, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment of BRD3 (Human)

  • 阳性对照
    • WB: HeLa, F2408 and NIH3T3 whole cell lysates. ICC/IF and FC: HeLa cells. IP: RIPA lysate of HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab50818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 0.5 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/50. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
IP Use at 5-1 µg/mg of lysate.
ICC/IF 1/10.

靶标

  • 组织特异性Ubiquitous.
  • 疾病相关Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • 序列相似性Contains 2 bromo domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRD 3 antibody
    • Brd3 antibody
    • BRD3_HUMAN antibody
    • Bromodomain containing 3 antibody
    • Bromodomain containing protein 3 antibody
    • Bromodomain-containing protein 3 antibody
    • KIAA0043 antibody
    • Open reading frame X antibody
    • ORFX antibody
    • RING3 like gene antibody
    • RING3-like protein antibody
    • RING3L antibody
    see all

Anti-BRD3 antibody [2088C3a] 图像

  • Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Anti Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 80 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Anti-Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    Anti-Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • Immunoflourescent staining of Hela cells using ab50818 Anti-BRD3 Antibody (1/10 dilution) as primary and AlexaFluor®488 Goat Anti-mouse IgG( 1/200 dilution )
  • Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa.
  • Flow Cytometric analysis using ab50818 (0.5ug). Secondary antibody used: AlexaFluor®488 Goat Anti-mouse IgG (0.25 μg)

Anti-BRD3 antibody [2088C3a] (ab50818)参考文献

ab50818 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEFs)
Loading amount 15 µg
Specification MEFs
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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提交于 Jan 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"