概述

  • 产品名称Anti-BRD2抗体
    参阅全部 BRD2 一抗
  • 描述
    山羊多克隆抗体to BRD2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Bromodomain Containing 2 (BRD 2) between the putative nuclear localization signal and the ET domain.

  • 阳性对照
    • HeLa cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab3718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/40000. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 22215678

靶标

Anti-BRD2 antibody 图像



  • Predicted band size : 110 kDa


    Sample: Hela cell total RIPA extract (10µg) Antibody: Affinity purified Goat anti-BRD 2 (ab3718) (diluted 1 / 20,000) CIP: Calf Intestinal Phosphatase Detection: ECL exposed for 1 minute.

Anti-BRD2 antibody (ab3718)参考文献

This product has been referenced in:
  • Dhar S  et al. Insights into role of bromodomain, testis-specific (Brdt) in acetylated histone H4-dependent chromatin remodeling in mammalian spermiogenesis. J Biol Chem 287:6387-405 (2012). ICC/IF ; Rat . Read more (PubMed: 22215678) »
  • Draker R  et al. A combination of H2A.Z and H4 acetylation recruits Brd2 to chromatin during transcriptional activation. PLoS Genet 8:e1003047 (2012). WB . Read more (PubMed: 23144632) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic fibroblasts)
Loading amount 15 µg
Specification embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Verified customer

提交于 Jan 22 2013

Thank you for contacting us.

Yes, ab3718 can recognise both the phosphorylated and unphosphorylated BRD2.

For ab113117, we recommend to use 1ug/ml (1:1000) in WB.

I hope this information is helpful to you. Please do not h...

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Thank you for contacting us.

The target protein got a number of phosphorserine and phosphothreonine residues which can increase the molecular weight of the protein.

I hope this information is helpful to you. Please do not hesitate ...

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Thank you for your enquiry. I have requested that the lab performs an alignment of the immunogen sequences with the rat homologs. Details of the degree of similarity is below: BRD2(rat) - GeneID: 294276 (NP_997660) The peptide immunogen w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"