Anti-BRD2抗体(ab111078)

概述

  • 产品名称Anti-BRD2抗体
    参阅全部 BRD2 一抗
  • 描述
    兔多克隆抗体to BRD2
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 675 and 725 of Human BRD2 (NP_005095.1).

  • 阳性对照
    • Human Breast Carcinoma, Colon Carcinoma and Prostate Carcinoma tissues; HeLa Cells.

性能

应用

Our Abpromise guarantee covers the use of ab111078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
ICC/IF 1/100 - 1/500. Formaldehyde-Fixed. Permeabilization with Triton-X 100 is recommended.

靶标

  • 功能May play a role in spermatogenesis or folliculogenesis.
  • 序列相似性Contains 2 bromo domains.
    Contains 1 ET domain.
  • 结构域One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRD 2 antibody
    • Brd2 antibody
    • BRD2_HUMAN antibody
    • Bromodomain containing 2 antibody
    • Bromodomain-containing protein 2 antibody
    • D6S113E antibody
    • DKFZp686N0336 antibody
    • Female sterile homeotic related gene 1 antibody
    • Female sterile homeotic related gene 1, mouse, homolog of antibody
    • FLJ31942 antibody
    • FSH antibody
    • FSRG1 antibody
    • KIAA9001 antibody
    • NAT antibody
    • O27.1.1 antibody
    • Really interesting new gene 3 protein antibody
    • RING3 antibody
    • RING3 PROTEIN antibody
    • RNF3 antibody
    see all

Anti-BRD2 antibody 图像

  • ab111078 at 1/250 dilution staining BRD2 in formaldehyde-fixed asynchronous HeLa cells by cytochemistry.
    Detection: Red fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1/100.
  • ab111078 at 1/250 dilution staining BRD2 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
    Detection: DAB staining.

Anti-BRD2 antibody (ab111078)参考文献

ab111078 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEFs)
Loading amount 15 µg
Specification MEFs
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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提交于 Jan 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"