概述

  • 产品名称Anti-BRCC45抗体
    参阅全部 BRCC45 一抗
  • 描述
    兔多克隆抗体to BRCC45
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 338 of Human BRCC45 (NP_004890).

  • 阳性对照
    • HepG2, HeLa and NIH-3T3 whole cell lysates; 293T, A431, H1299, HeLaS3, MOLT4 and Raji cell lysates ICC/IF: HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab95984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.
  • 组织特异性Expressed in all cell lines examined. Highly expressed in placenta.
  • 序列相似性Belongs to the BRE family.
  • 结构域Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.
  • 细胞定位Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6030405P19Rik antibody
    • AI429776 antibody
    • B830038C02Rik antibody
    • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
    • brain and reproductive organ expressed protein antibody
    • Brain and reproductive organ-expressed protein antibody
    • BRCA1 A complex subunit BRE antibody
    • BRCA1-A complex subunit BRE antibody
    • BRCA1/BRCA2 containing complex subunit 4 antibody
    • BRCA1/BRCA2 containing complex subunit 45 antibody
    • BRCA1/BRCA2-containing complex subunit 45 antibody
    • BRCC4 antibody
    • BRCC45 antibody
    • bre antibody
    • BRE_HUMAN antibody
    see all

Anti-BRCC45 antibody 图像

  • All lanes : Anti-BRCC45 antibody (ab95984) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
  • Immunofluorescence analysis of BRCC45 in paraformaldehyde fixed HeLa, using ab95984 at 1/200 dilution. Lower image merged with DNA probe.
  • Anti-BRCC45 antibody (ab95984) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 47 kDa

Anti-BRCC45 antibody (ab95984)参考文献

ab95984 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95984.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"