RabMAb

Anti-BRCC36抗体[EPR4366] (ab108411)

概述

  • 产品名称Anti-BRCC36抗体[EPR4366]
    参阅全部 BRCC36 一抗
  • 描述
    兔单克隆抗体[EPR4366] to BRCC36
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human BRCC36.

  • 阳性对照
    • WB: HeLa, MCF7, SKBR-3, and 293T cell lysates Flow Cyt: permeabilized HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab108411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
    • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors.
    • 疾病相关A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.
    • 序列相似性Belongs to the peptidase M67A family. BRCC36 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • 细胞定位Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690).
    • Information by UniProt
    • 数据库链接
    • 别名
      • BRCA1-A complex subunit BRCC36 antibody
      • BRCA1/BRCA2 containing complex subunit 3 antibody
      • BRCA1/BRCA2 containing complex subunit 36 antibody
      • BRCA1/BRCA2-containing complex subunit 3 antibody
      • BRCA1/BRCA2-containing complex subunit 36 antibody
      • Brcc3 antibody
      • BRCC3_HUMAN antibody
      • BRCC36 antibody
      • BRISC complex subunit BRCC36 antibody
      • C6.1A antibody
      • Chromosome X open reading frame 53 antibody
      • CXorf53 antibody
      • Lys-63-specific deubiquitinase BRCC36 antibody
      • OTTHUMP00000061450 antibody
      see all

    Anti-BRCC36 antibody [EPR4366] 图像

    • All lanes : Anti-BRCC36 antibody [EPR4366] (ab108411) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 36 kDa
    • Flow cytometric analysis of BRCC36 in permeabilized HeLa cells, using ab108411 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).

    Anti-BRCC36 antibody [EPR4366] (ab108411)参考文献

    ab108411 has not yet been referenced specifically in any publications.

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