The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
The novel BRCAA1 protein’s epitope, SSKKQKRSHK, was mapped to the cytoplasm of MCF7 cells. Immunohistochemistry analysis has shown that the antigen epitope exhibits positive expression in 65% of breast cancer specimens. Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.