概述

性能

应用

Our Abpromise guarantee covers the use of ab9143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 12915460
IP Use a concentration of 1 - 4 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 380 kDa (predicted molecular weight: 380 kDa).

靶标

  • 功能Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.
  • 组织特异性Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
  • 疾病相关Defects in BRCA2 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in BRCA2 are the cause of pancreatic cancer type 2 (PNCA2) [MIM:613347]. It is a malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, connective tissue, and lymphatic tissue.
    Defects in BRCA2 are a cause of susceptibility to breast-ovarian cancer familial type 2 (BROVCA2) [MIM:612555]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
    Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    Defects in BRCA2 are a cause of glioma type 3 (GLM3) [MIM:613029]. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
  • 序列相似性Contains 8 BRCA2 repeats.
  • 翻译后修饰Phosphorylated by ATM upon irradiation-induced DNA damage.
    Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRCA 2 antibody
    • BRCA1/BRCA2 containing complex subunit 2 antibody
    • Brca2 antibody
    • BRCA2, DNA repair associated antibody
    • BRCA2_HUMAN antibody
    • BRCC 2 antibody
    • BRCC2 antibody
    • Breast and ovarian cancer susceptibility gene early onset antibody
    • breast and ovarian cancer susceptibility protein 2 antibody
    • Breast cancer 2 early onset antibody
    • Breast Cancer 2 tumor suppressor antibody
    • Breast cancer susceptibility protein BRCA2 antibody
    • Breast cancer type 2 susceptibility protein antibody
    • BROVCA2 antibody
    • FACD antibody
    • FAD 1 antibody
    • FAD antibody
    • FAD1 antibody
    • FANCB antibody
    • FANCD 1 antibody
    • FANCD antibody
    • FANCD1 antibody
    • FANCD1 gene antibody
    • Fanconi anemia complementation group D1 antibody
    • Fanconi anemia group D1 protein antibody
    • GLM3 antibody
    • mutant BRCA2 antibody
    • OTTHUMP00000018803 antibody
    • OTTHUMP00000042401 antibody
    • PNCA2 antibody
    • XRCC11 antibody
    see all

Anti-BRCA2 antibody 图像

  • ab9143 immunoprecipitating BRCA2 at 6 µg per reaction.

    Lane 1: ab9143 in HEK2936 cell lysate.

    Lane 2: Rabbit polyclonal antibody (ab123491) used instead of ab9143 in 293T cell lysate

    Lane 3 (control): Secondary antibody only control

    For blotting, ab9143 immunoprecipitated BRAC2 at 1 µg/ml. Detected by Chemiluminescence.

Anti-BRCA2 antibody (ab9143)参考文献

This product has been referenced in:
  • Orthwein A  et al. A mechanism for the suppression of homologous recombination in G1 cells. Nature 528:422-6 (2015). Read more (PubMed: 26649820) »
  • Suzuki S  et al. Inhibition of post-translational N-glycosylation by HRD1 that controls the fate of ABCG5/8 transporter. Sci Rep 4:4258 (2014). Human . Read more (PubMed: 24584735) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 50 µg
Specification 293T
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Verified customer

提交于 Nov 02 2016

Thanks for your inquiry. I have copied below some details on transferring large proteins. Detailed instructions for the transfer process can be found on the websites of the manufacturers of transfer apparatus, and will vary depending on the ...

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Thank you for your enquiry and your interest in our products. We would like to confirm that the two product are the same and from the same originator. The only difference is that ab1292 is Rabbit polyclonal to BRCA2 (Agarose) suitable for only IP ap...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"