概述

  • 产品名称Anti-BRCA2抗体
    参阅全部 BRCA2 一抗
  • 描述
    兔多克隆抗体to BRCA2
  • 特异性ab27976 recognises BRCA2.
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide mapping to the N terminus of human BRCA2.

  • 阳性对照
    • WB: mouse cell line SV-T2 lysate

性能

应用

Our Abpromise guarantee covers the use of ab27976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 384 kDa.Can be blocked with BRCA2 peptide (ab69799).
IP Use at an assay dependent concentration. PubMed: 24210700
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.
  • 组织特异性Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
  • 疾病相关Defects in BRCA2 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in BRCA2 are the cause of pancreatic cancer type 2 (PNCA2) [MIM:613347]. It is a malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, connective tissue, and lymphatic tissue.
    Defects in BRCA2 are a cause of susceptibility to breast-ovarian cancer familial type 2 (BROVCA2) [MIM:612555]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
    Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    Defects in BRCA2 are a cause of glioma type 3 (GLM3) [MIM:613029]. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
  • 序列相似性Contains 8 BRCA2 repeats.
  • 翻译后修饰Phosphorylated by ATM upon irradiation-induced DNA damage.
    Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRCA 2 antibody
    • BRCA1/BRCA2 containing complex subunit 2 antibody
    • Brca2 antibody
    • BRCA2, DNA repair associated antibody
    • BRCA2_HUMAN antibody
    • BRCC 2 antibody
    • BRCC2 antibody
    • Breast and ovarian cancer susceptibility gene early onset antibody
    • breast and ovarian cancer susceptibility protein 2 antibody
    • Breast cancer 2 early onset antibody
    • Breast Cancer 2 tumor suppressor antibody
    • Breast cancer susceptibility protein BRCA2 antibody
    • Breast cancer type 2 susceptibility protein antibody
    • BROVCA2 antibody
    • FACD antibody
    • FAD 1 antibody
    • FAD antibody
    • FAD1 antibody
    • FANCB antibody
    • FANCD 1 antibody
    • FANCD antibody
    • FANCD1 antibody
    • FANCD1 gene antibody
    • Fanconi anemia complementation group D1 antibody
    • Fanconi anemia group D1 protein antibody
    • GLM3 antibody
    • mutant BRCA2 antibody
    • OTTHUMP00000018803 antibody
    • OTTHUMP00000042401 antibody
    • PNCA2 antibody
    • XRCC11 antibody
    see all

Anti-BRCA2 antibody 图像

  • ab27976 staining BRCA2 in a xenograft MDA-MB-231 of human breast cancer by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% BSA for 30 minutes at 25°C and then incubated with ab27976 at a 1/50 dilution for 16 hours at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview

Anti-BRCA2 antibody (ab27976)参考文献

This product has been referenced in:
  • Mundia MM  et al. Nascent DNA synthesis during homologous recombination is synergistically promoted by the rad51 recombinase and DNA homology. Genetics 197:107-19 (2014). Read more (PubMed: 24583581) »
  • Reuter M  et al. BRCA2 diffuses as oligomeric clusters with RAD51 and changes mobility after DNA damage in live cells. J Cell Biol 207:599-613 (2014). Read more (PubMed: 25488918) »

See all 4 Publications for this product

Product Wall

We do offer two anti- BRCA2 products which are guaranteed for use in mouse samples. Ab27976 is against the N-terminal region and has been referenced in publication with mouse samples. This product is tested in IHC-P and is covered by our six month Abpr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast cancer xenograft)
Specification breast cancer xenograft
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 22 2012

I have just received feedback from the originator of ab27976 and was informed that the western blot was done with a mouse cell line SV-T2 lysate, we do not have an image currently. The homology of the immunogen between human and mouse is ~77%. I ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"