The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
BRCA1 Associated Protein, BRAP2 (Impedes mitogenic signal propogation; IMP) is mainly cytoplasmic and binds to the NLS motifs of BRCA1 with similar specificity to that of importin alpha. BRAP2 may function as a cytoplasmic retention protein and play a role in regulating transport of nuclear proteins. More recently, the protein has been shown to impede signal transmission from Raf to MEK.
3µg/ml ab95179 were used to immunoprecipitate BRAP from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with a different BRAP-specific antibody. Detection: chemiluminescence with exposure time of 30 seconds.